CD178 Mouse anti-Human, Unlabeled, Clone: G247-4, BD

Description

• Fas (APO-1, CD95) is a 45 kD cell surface protein that mediates apoptosis when crosslinked with agonistic anti-Fas antibodies or Fas ligand (FasL)
• Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung
• FasL is a member of the TNF cytokine family that induces apoptosis by binding to Fas, its cell-surface receptor
• FasL may exist in both membrane and soluble forms and expressed on activated T cells, NK cells, and other “immunologically privileged” sites
• Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development
• G247-4 recognizes human FasL
• It recognizes both the membrane bound (FasL) and soluble (sFasL) forms
• A recombinant protein containing the external domain of human FasL was used as immunogen
• FasL and sFasL migrate at reduced molecular weights of 40 and 26 kD, respectively
• However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al
• For example, FasL may migrate as a doublet of 40 and 42 kD.