SREBP-1 Mouse, Unlabeled, Clone: IGG-2A4, BD

Description

• SREBP-1 and -2 (sterol-regulatory element binding proteins-1 and -2) are transcription factors which participate in the control of cholesterol homeostasis
• SREBPs proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol
• Upon activation of SREBP-1 or -2, an ∽480-500 amino acid, N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis
• Proteases which cleave SREBPs have been identified and include SCA (SREBP-cleavage activity), as well as a key regulator of apoptotic pathways, caspase-3
• SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes
• However, sterol-regulated vs
• apoptosis-associated cleavage of SREBP proteins appears to be independantly regulated
• On SDS-PAGE, sterol-regulated cleavage fragments of SREBP proteins migrate more slowly (i.e., higher molecular weight) than do staurosporin-induced fragments
• In addition, staurosporin-induced SREBP cleavage products may appear as a doublet, with the upper band representing a phosphorylated form of SREBP
• On SDS-PAGE, full length, precursor forms of SREBP-1 and -2 migrate at ∽125kDa, while proteolytic cleavage fragments may be observed as a cluster of bands between 60 - 70kDa
• The IgG-2A4 antibody recognizes human and hamster SREBP-1
• The antibody recognizes the N-terminal (basic helix-loop-helix) domain of human SREBP-1
• A fusion protein containing N-terminal amino acids 301-407 (the bHLH/leucine zipper domain), was used as immunogen
• The antibody recognizes both the 125kDa precursor and 60-70kDa mature, cleaved forms of SREBP-1
• Host Species: Mouse Clone: IgG-2A4 Isotype: IgG1, κ Species Reactivity: Human Immunogen: Human SREBP-1 aa
• 301-407 Immunoprecipitation, Western Blotting