p130Cas (pY249) Mouse anti-Human, Unlabeled, Clone: J169-757.12.2, BD

Description

• Description p47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses
• The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains
• v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate)
• The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk
• Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk
• This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus
• Since p130Cas also associates with v-Src, it may be a v-Src substrate
• Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule
• The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas
• The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively
• Western Blotting