PARP Mouse anti-Human, Unlabeled, Clone: 4C10-5, BD

Description

• PARP [Poly(ADP-ribose) polymerase] is a 113kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself
• The catalytic activity of PARP is increased in non-apoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage
• Additionally, PARP is a target of the caspase protease activity associated with apoptosis
• During apoptosis, PARP is cleaved from a 113kDa intact form into smaller 89kDa and 24kDa fragments
• This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function
• Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis
• The 4C10-5 antibody recognizes both the intact 113kDa form and 89kDa fragment of PARP
• The 4C10-5 antibody has been reported to recognize both native and denatured PARP
• Purified human PARP was used as the immunogen and the antibody reported to react with an epitope located in the NAD binding domain
• In dot blot assays, the antibody reacts with the native enzyme in the presence or absence of bound DNA as well as after synthesis of covalently linked poly (ADP-ribose)
• The 4C10-5 antibody is routinely tested by western blot analysis of untreated Jurkat T cells and Jurkat T cells induced to undergo apoptosis
• This antibody is routinely tested by Western blot analysis and immunofluorescent imaging
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature
• Host Species: Mouse Clone: 4C10-5 Isotype: IgG1 κ Species Reactivity: Human Immunogen: Human PARP Bioimaging, Dot Blotting, Flow Cytometry, Immunoprecipitation, Intracellular Staining, Western Blotting