MAP2 Mouse, Unlabeled, Clone: AP20, BD

Description

• Microtubule-associated protein 2 (MAP2) is a neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules
• It is expressed in the cell body and dendrites of neurons, but is absent in neuronal processes
• The expression of MAP2 is developmentally regulated, and there are multiple high and low molecular weight isoforms, all derived from one MAP2 gene
• MAP2a, MAP2b, and MAP2c are the three major MAP2 isoforms
• The high molecular weight MAP2 isoforms, MAP2a and MAP2b consist of a large projection arm and a short microtubule binding domain
• The low molecular weight MAP-2 isoform, MAP2c, lacks most of the projection arm of the high molecular weight isoforms
• MAP2c is the earliest expressed MAP2 and is derived by alternative splicing MAP2a and MAP2b are considered to be adult expressed MAPs, with MAP2a expression occuring later in development than MAP2b expression
• MAP2a is thought to result from a post-translational modification of MAP2b
• High molecular weight MAP2 isoforms (2a and 2b) migrate as a doublet at a molecular weight of >300kDa
• The low molecular weight MAP2 isoform 2c migrates at 70kDa
• The Ap-20 antibody recognizes high molecular weight MAP2 isoforms MAP2a and MAP2b
• Specifically, it has been shown to recognize MAP2a and MAP2b from human, bovine, rat, frog, and quail cells and tissues
• Ap-20 does not recognize the low molecular weight MAP2 isoforms (MAP2c) or other microtubule proteins
• Ap-20 reacts with an epitope between amino acids 997-1332 of high molecular weight MAP2 isoforms.4 Bovine MAP2 was used as immunogen
• Host Species: Mouse Clone: Ap20 Isotype: IgG1 Species Reactivity: Rat Immunogen: Cow MAP2 Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting