p73 Mouse anti-Human, Unlabeled, Clone: GC-15, BD

Description

• p53 is a tumor suppressor which acts as an S-phase checkpoint for DNA damage
• The gene for p53 is the most commonly mutated gene identified in human cancers
• Recently, a new member of the p53 family, p73, was identified
• p73 is structurally homologous to p53 in several regions, including the p53 N-terminal transactivation domain and C-terminal oligomerization domains, as well as the region corresponding to the p53 DNA-binding domain
• When overexpressed, p73 can promote p53-like functions, including induction of apoptosis and induction of transcription from p53-responsive promoters such as p21
• Despite structural and apparent functional homology, data suggests that these proteins may have distinct functions as well
• For example, viral oncoproteins such as Adenovirus E1B 55K and HPV E6, which bind to and thus inactivate p53 during the process of transformation, do not bind to p73
• In addition, unlike p53, p73 expression is not induced by DNA damage, e.g
• UV irradiation
• Several p73 splice variants have been identified, including α (full-length), β (missing exon 13), γ (missing exon 11) and δ (missing exons 11, 12 and 13)
• Two hybrid analysis has shown variable interaction(s) between these isoforms in vitro
• Many types of normal, tumor and virally-transformed cell lines express detectable levels of p73; however, the relative expression of p73 isoforms, as well as their functional activity, appears to be differentially regulated in various cell types
• p73α and β isoforms migrate at molecular weights of approximately 80kDa (α) and 70kDa (β), respectively
• The GC-15 antibody reacts with human p73α and β
• A fusion protein containing amino acids 380-367 of human p73α was used as immunogen
• Immunoprecipitation, Western Blotting