Perforin Mouse anti-Human, PE, Clone: ΔG9, BD

Description

• Perforin has a key role in cell-mediated cytotoxicity
• It is a 70kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells
• CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases
• Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell
• In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis
• The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions
• Clone δG9 reacts with human and bovine perforin
• It does not cross-react with mouse perforin
• Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections, and has also been published for flow cytometric analysis
• Clone 27-35 is an isotype control monoclonal antibody specific for hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl)
• This hapten is not expressed on human cells or human cell lines
• Host Species: Mouse Clone: δG9, 27-35 Isotype: IgG2b, κ Species Reactivity: Human Immunogen: Purified Granules from the Human Lymphoma Cell Line YT Flow Cytometry