CD56 Mouse anti-Human, BD IMag-DM, Clone: NCAM16.2, BD

Description

• BD IMag™ Anti-Human CD56 Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces
• These particles are optimized for the positive selection or depletion of the CD56-bearing leukocytes using the BD™ IMagnet
• CD56, also known as the neural cell adhesion molecule (N-CAM), is expressed in many different cell and tissue types
• In normal donors, it is found on NK and NK-T cells
• The NCAM16.2 mAb has been reported to cross-react with lymphocytes of the rhesus macaque
• BD IMag™ Anti-Human CD56 magnetic particles have been reported to effectively separate CD56-bearing cells of rhesus macaque blood
• For enrichment of NK cells without NK-T cells, the use of BD™ IMag Anti-Human CD3 Magnetic Particles - DM (Cat
• no
• 552593) are suggested for depleting T lymphocytes before separation of CD56-positive leukocytes
• Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD56 Magnetic Particles - DM according to the Magnetic Labeling Protocol
• This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™
• Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction)
• The tube is then removed from the magnetic field for resuspension of the positive fraction
• The separation is repeated twice to increase the purity of the positive fraction
• The magnetic separation steps are diagrammed in the Separation Flow Chart
• After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications, such as by flow cytometry.