LAT (pY171) Mouse anti-Human, Unlabeled, Clone: I58-1169, BD

Description

• Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation
• TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70
• Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules
• LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates
• LAT is expressed as 36- and 38kDa forms that result from post-translational modification, and as a 42kDa form that results from alternative splicing
• LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation
• Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCγ1, and the p85 subunit of PI3K
• Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development
• The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads
• Immunohistochemistry, Western Blotting