CD247 (pY142) Mouse, Alexa Fluor 488, Clone: K25-407.69, BD

Description

• The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules
• The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d
• Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and ζ ) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop
• CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes
• After antigen recognition by the TCR, CD3 z is the primary intracellular signal transducing subunit
• It contains three ITAMs ( I mmunoreceptor T yrosine-based A ctivation M otifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation
• The molecular weight of CD3 z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors
• Upon phosphorylation, the CD3 z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa
• The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3 z (CD247) .