MEK1 Mouse, Alexa Fluor 647, Clone: 25/MEK1, BD

Description

• MEK1 ( M apK/ E RK K inase ) is a 45-kDa member of the MEK family of dual specificity kinases
• MEK is activated by a variety of cellular serine/threonine kinases including c-Raf, A-Raf, c-mos, and MEK Kinase-1
• Activated MEK phosphorylates MAP kinase (ERK) at threonine and tyrosine residues
• This results in activation of ERK and its signaling pathway
• MEK is highly specific for ERK and various MEKs preferentially phosphorylate individual ERK isoforms
• MEK1 only activates ERK1 and ERK2
• This specificity may result from variations in ERK regions that are known as the phosphorylation lip and kinase backbone
• MEK's localization is cytoplasmic, but mitogenic stimulation induces a mass translocation to the nucleus
• Mechanisms behind this nuclear translocation remain unknown
• However, MEK contains an N-terminal nuclear export signal (NES) that mediates its rapid exodus from the nucleus and restores its unstimulated cellular distribution
• The 25/MEK1 monoclonal antibody recognizes MEK1, regardless of phosphorylation status
• The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure)
• Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.