SHP2 (pY542) Mouse, PE, Clone: L99-921, BD

Description

• SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs)
• SHP2 reportedly contains two SH2 domains, both of which are N-terminal to the PTP catalytic domain
• SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression
• The expression of SHP2 has been reported to be highest in brain, heart, and kidney
• SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580
• Phosphorylation of Y542 creates an interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an interaction at the C-terminal that stimulates PTP activity
• The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2)
• The homologous phosphorylation site in the PTP2Ci splice variant (isoform 1) is Y546
• The specificity of this antibody was validated by confirming, using western blot analysis, that RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542)
• Host Species: Mouse Clone: L99-921 Isotype: IgG1 κ Species Reactivity: Human Immunogen: Phosphorylated Human SHP2 Peptide Intracellular Staining