CD4 Rat anti-Mouse, V450, Clone: RM4-5, BD

Description

• The RM4-5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells
• CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells
• CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm
• CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells
• It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck
• Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone
• The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450nm
• Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates
• Host Species: Rat Clone: RM4-5 Isotype: IgG2a κ Species Reactivity: Mouse Immunogen: Mouse Thymocytes (BALB/c) Flow Cytometry