CD4 Rat anti-Mouse, V500, Clone: RM4-5, BD

Description

• The RM4-5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells
• CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells
• CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm
• CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells
• It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck
• Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone
• The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser with an Ex max of 415 nm and Em Max at 500 nm
• BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
• For more information on BD Horizon V500, visit bdbiosciences.com/colors
• When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
• Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different V500 reagents (e.g
• CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents
• Flow Cytometry