CD8a Rat anti-Mouse, V500, Clone: 53-6.7, BD

Description

• The 53-6.7 antibody has been reported to react with the 38kDa α and 34kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested
• The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes
• A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent)
• Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b
• It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals
• CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells
• It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 lck)
• The CD8 α and α' chains arise from alternatively spliced messengers of a single CD8a gene
• The longer α form associates with p56 (lck) via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b
• The truncated α' chain is unable to associate with p56 (lck), and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation
• In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes
• The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis
• The antibody is conjugated to BD Horizon V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser with an Ex max of 415 nm and Em Max at 500 nm
• BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
• When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
• Due to spectral differences between cells and beads in this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different V500 reagents (e.g
• CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents
• Host Species: Rat Clone: 53-6.7 Isotype: IgG2a κ Species Reactivity: Ms Immunogen: Mouse thymus / spleen Cells Flow Cytometry