S6 (pS235/pS236) Mouse anti-Human, V450, Clone: N7-548, BD

Description

• Ribosomal protein S6 ( ∽29 kDa calculated and ∽32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins
• The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells
• S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6
• The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression
• These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens
• The S6 ribosomal protein can be dephosphorylated in growth-arrested cells
• The N7-548 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S235 and S236
• The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm
• Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.