BDB562055

Ly-49C and Ly-49I Mouse anti-Mouse, FITC, Clone: 5E6, BD

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 BDB562055-Each-of-1 In Stock ₹ 18,309.84

BDB562055 - Each of 1

₹ 18,309.84

In Stock

Quantity

1

Base Price: ₹ 18,309.84

GST (18%): ₹ 3,295.771

Total Price: ₹ 21,605.611

Antigen

Ly-49C and Ly-49I

Classification

Monoclonal

Concentration

0.5mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

Activated mouse NK cells

Quantity

0.1 mg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

5.00E+06

Conjugate

FITC

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG2a κ

Description

  • The 5E6 (also known as clone SW5E6) antibody reacts with Ly-49C[BALB], Ly-49C[B6], Ly-49C[NZB], and Ly-49I[B6], inhibitory receptors which are expressed on subsets of natural killer (NK) cells and NK-1.1+ (or DX5+) T lymphocytes (NK-T cells) in all strains tested except C57BR and RIII, on a population of memory CD8+ T lymphocytes and NK1.1+ γδ T cells in C57BL/6 mice, and on a distinct subset of B-1 cells of BALB/c and C57BL/6 mice
  • The proportion of NK T cells expressing Ly-49C/I is higher (2-5 fold) in thymus than in liver (immature and mature NK T cells, respectively), and there is evidence that the down-regulation of Ly-49 receptor expression is necessary for normal NK T-cell development
  • Most NK cells express a single allele of Ly-49C, although occasionally they may express more than one allele
  • The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD) that bind to MHC class I alloantigens
  • The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers
  • The 5E6 antibody is specific for the Ly-49C CRD
  • The Ly-49C[BALB] and Ly-49C[B6] alloantigens bind to MHC class I antigens of the b, d, k, and s haplotypes, and the 5E6 antibody blocks this binding
  • Binding of Ly-49C[BALB]- and Ly-49C[B6]- expressing transfectants to lymphoblasts of H-2[f], H-2[q], H-2[r], and H-2[v] strains has also been detected
  • Ly-49I[B6] transfectants bind H-2[r] lymphoblasts and bind much more weakly to the b, d, k, q, s, and v haplotypes
  • The levels of the Ly-49 inhibitory receptors are down-regulated by their ligands in vivo , and the various levels of expression of an Ly-49 inhibitory receptor may affect the specificity of NK cells
  • Ly-49C is specifically downregulated in the presence of H-2K[b] class I molecules (one of the Ly-49C ligands)
  • Ly-49C[+] and/or Ly-49I[+] cells mediate allogeneic and hybrid resistance to H-2d bone marrow transplantation
  • In vitro and in vivo I mmunoreceptor T yrosine-based I nhibitory M otif)
  • The epitope recognized by this antibody on Ly49C may be masked on freshly isolated primary NK cells due to cis interactions with MHC class I molecules
  • This observation has been reported for other Ly49C monoclonal antibodies that bind to the same structural region.

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Antigen:
Ly-49C and Ly-49I

Classification:
Monoclonal

Concentration:
0.5mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Activated mouse NK cells

Quantity:
0.1 mg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
5.00E+06

Conjugate:
FITC

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ

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Antigen:
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Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
IL-2-dependent mouse T-cell line CTL-L

Quantity:
25 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
D7

Conjugate:
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Host Species:
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Purification Method:
Affinity Purified

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Concentration:
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Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Immunogen:
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Quantity:
25 μg

Primary or Secondary:
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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
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Conjugate:
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Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2b κ

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Monoclonal

Concentration:
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Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
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Quantity:
25 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
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Clone:
PK136

Conjugate:
PE-Cyanine7

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG2a κ