CD123 Mouse anti-Human, PE-CF594, Clone: 7G3, BD

Description

• The 7G3 monoclonal antibody specifically reacts with human CD123, the 70 kDa IL-3 Receptor α (IL-3Rα) chain
• CD123 associates with CD131, the 120-140 kDa Common β chain to form the IL-3 Receptor Complex
• CD131 is shared with the receptors for interleukins IL-5 and GM-CSF
• IL-3Rα is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation
• It is also expressed by mast cells, macrophages and a CD5+ B cell subset
• This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors
• In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration
• This antibody has been reported to be useful for immunoprecipitation, Western blot and immunofluorescent staining for flow cytometry
• At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).