CD123 Mouse anti-Human, Brilliant Violet 650, Clone: 7G3, BD

Description

• The 7G3 monoclonal antibody specifically reacts with human CD123, the 70 kDa IL-3 Receptor α (IL-3Rα) chain
• CD123 associates with CD131, the 120-140 kDa Common β chain to form the IL-3 Receptor Complex
• CD131 is shared with the receptors for interleukins IL-5 and GM-CSF
• IL-3Rα is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation
• It is also expressed by mast cells, macrophages and a CD5+ B cell subset
• This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors
• In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration
• This antibody has been reported to be useful for immunoprecipitation, Western blot and immunofluorescent staining for flow cytometry
• At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.