CD279 Hamster anti-Mouse, PE-CF594, Clone: J43, BD

Description

• Description The J43 monoclonal antibody specifically binds to CD279 (PD-1), a 50-55-kDa glycoprotein encoded by the Pdcd1 gene of the CD28 family of the Ig superfamily
• The expression of Pdcd1 mRNA and PD-1 protein is tightly regulated
• PD-1 is transiently expressed on CD4-CD8thymocytes, it is upregulated on some cell lines upon induction of apoptosis, it is induced on thymocytes and splenic T and B lymphocytes after stimulation through their antigen receptors, and it is induced on activated myeloid cells
• In addition, Pdcd1 mRNA is transiently expressed in developing B lymphocytes at the pro-B-cell stage
• The presence of an ITIM ( yrosine-based I nhibitory M otif) on PD-1's intracytoplasmic region and the development of splenomegaly and breakdown of peripheral tolerance in PD-1[-/-] mice suggest that PD-1 may be involved in the negative regulation of immune responses
• The PD-1 ligands, B7-H1 (also known as PD-L1) and B7-DC (PD-L2), are members of the B7 family of the Ig superfamily
• The J43 antibody blocks the binding of PD-1 to its two ligands
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).