CD279 (PD-1) Mouse anti-Human, Brilliant Violet 510, Clone: EH12.1, BD

Description

• The EH12.1 monoclonal antibody specifically binds to CD279
• CD279 is an immunoregulatory receptor that is expressed on activated T cells, B cells and myeloid cells and contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region
• Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms
• PD-L1 and PD-L2 are ligands of CD279 and are members of the B7 gene family
• Interaction of CD279:PD-Ligands results in inhibition of T cell proliferation and cytokine secretion
• Reports suggest that the B7/CTLA-4 pathway functions primarily to attenuate, limit, and/or terminate naοve T-cell activation in secondary lymphoid organs
• The PD-ligand:CD279 pathway, on the other hand, may primarily attenuate, limit, and/or terminate T-, B-, and myeloid cell activation/effector function at sites of inflammation in the periphery
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set
• BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.