IKKγ (pS376) Mouse anti-Human, PE, Clone: N19-39, BD

Description

• The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKγ (pS376)
• IKKγ is also known an NEMO (NF-κ-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene
• The nuclear transcription factor κ-B, NF-κB, is controlled by interaction with an inhibitory subunit, IκB, which restricts NF-κB to the cytoplasm
• Following stimulation by various cytokines or other stimuli, IκB becomes degraded and NF-κB is released to the nucleus
• The release of IκB from NF-κB is thought to be a critical point in the activation of NF-κB signal pathways
• Activated NF-κB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways
• A group of proteins form an NF-κB regulatory complex, or signalsome
• Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (also called IKK-1 and IKK-2), which phosphorylate critical residues of IκB, thus targeting it for subsequent degradation
• The IKK complex contains similar amounts of IKKα, IKKβ as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ
• IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK)4 and MEKK1
• IKKβ has been shown to phosphorylate IKKγ at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1
• IKKγ has an essential regulatory role in the IKK complex and is required for the activation of the NF-κB pathway in response to multiple cellular stimuli
• IKKγ isoforms migrate as a doublet of ∽50-55kDa upon Western blot analysis
• Host Species: Mouse Clone: N19-39 Isotype: IgG1, κ Species Reactivity: Human Immunogen: Phosphorylated Human IKKγ Peptide Flow Cytometry