CD56 Mouse anti-Human, Brilliant Violet 605, Clone: NCAM16.2, BD

Description

• The NCAM16.2 monoclonal antibody specifically binds to human CD56
• It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39
• The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes
• It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes
• CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity
• CD56 antigen density on NK lymphocytes increases upon cellular activation
• The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis
• CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells
• This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.