FOXP3 Rat anti-Mouse, Brilliant Violet 421, Clone: MF23, BD

Description

• The MF23 monoclonal antibody specifically binds to mouse Foxp3
• Foxp3 is a 50-55 kDa protein also known as Forkhead box p3, JM2, or IPEX
• It is a member of the forkhead or winged helix family of transcription factors and is specifically expressed by T regulatory (Treg) cells
• Foxp3 has been reported to be a key regulatory protein for Treg cell development and function
• Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN- γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR
• It has been found that the mutation of Foxp3 is responsible for “scurfy” mice
• When overexpressed, Foxp3 leads to poor T cell proliferation and activation
• Immunoblotting with MF23 antibody has confirmed it recognizes an epitope between 1-87 amino acids in the N-terminal domain
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.