CD16/CD32 Rat anti-Mouse, Brilliant Violet 605, Clone: 2.4G2, BD

Description

• The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcγIII and FcγII receptors
• It has also been reported to bind the FcγI receptor (CD64) via its Fc domain
• 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, receptors
• CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes
• This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment
• Fluorescent dye interactions may cause staining artifacts which may affect data interpretation
• The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions
• More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat
• No
• 563794).