Ig Goat anti-Mouse, Brilliant Violet 421, Polyclonal, BD

Description

• BD Horizon™ BV421 Goat Anti-Mouse Ig is intended to be a second-step reagent for staining of cells pre-stained with primary mouse Ig antibodies
• It is reactive with the heavy and light chains of mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes
• Minimal background staining of human and rat leucocytes occurs in the absence of a primary mouse antibody
• In addition, the BD Horizon BV421 Goat Anti-Mouse Ig stains mouse peripheral B lymphocytes with little non-specific staining of other splenic leucocytes
• Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining cells pre-stained with mouse Ig primary antibodies
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.