CD138 Rat anti-Mouse, Brilliant Violet 605, Clone: 281-2, BD

Description

• The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules
• Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization
• It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells
• It is thus implicated in mediating B cell-matrix interactions
• CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications
• The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein
• Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon™ BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon™ BV605 is a tandem fluorochrome of BD Horizon™ BV421 and Cy™3.5
• Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon™ PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon™ BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.