CD138 Mouse anti-Human, Brilliant Violet 421, Clone: MI15, BD

Description

• The MI15 monoclonal antibody specifically binds to CD138 (Syndecan-1), an 85-92 kDa single chain transmembrane protein, strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations
• It is also expressed on pre-B cells, immature B cells, and plasma cells, but not on mature circulating B-lymphocytes
• Syndecan-1 is a member of the family of transmembrane heparan sulfate proteoglycans
• It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial and neural cells
• CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III
• It is considered an extracellular matrix receptor that may serve as a co-receptor for fibroblast growth factor and related molecules
• Monoclonal antibody MI15 blocks the binding of clone B-B4 but not clone DL-101 (other anti-syndecan-1 antibodies) by flow cytometric analysis
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.