CD86 Mouse anti-Human, Brilliant Violet 711, Clone: 2331 (FUN-1), BD

Description

• The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells
• Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (anti-CD86, Cat
• No
• 555663) antibodies specifically recognize the same molecule, they react with different epitopes
• CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in co-stimulation of T cells in primary immune response
• The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.