CD80 Mouse anti-Human, Brilliant Violet 650, Clone: L307.4, BD

Description

• The L307.4 monoclonal antibody specifically binds to B7/BB1, a 60 kDa transmembrane glycoprotein that was clustered as CD80 in the Fifth International Workshop on Human Leukocyte Differentiation Antigens
• CD80, a member of the Ig supergene family, is expressed on activated B cells, T cells, macrophages, and dendritic cells
• It is the ligand for two molecules expressed on T cells, CD28 and CD152 (CTLA-4)
• CD80 is also expressed on activated CD4-positive and CD8-positive T cells, appearing late after activation suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway
• The binding of CD28 by anti-CD28 or by CD80 results in T-cell activation and a signal for IL-2 production
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.