CD80 Mouse anti-Human, Brilliant Violet 421, Clone: L307.4, BD

Description

• The L307.4 monoclonal antibody specifically binds to B7/BB1, a 60 kDa transmembrane glycoprotein that was clustered as CD80 in the Fifth International Workshop on Human Leukocyte Differentiation Antigens
• CD80, a member of the Ig supergene family, is expressed on activated B cells, T cells, macrophages, and dendritic cells
• It is the ligand for two molecules expressed on T cells, CD28 and CD152 (CTLA-4)
• CD80 is also expressed on activated CD4-positive and CD8-positive T cells, appearing late after activation suggesting that activated T cells may be capable of autocrine costimulation via the CD28 activation pathway
• The binding of CD28 by anti-CD28 or by CD80 results in T-cell activation and a signal for IL-2 production
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.