CD158A Mouse anti-Human, Brilliant Violet 711, Clone: HP-3E4, BD

Description

• The HP-3E4 monoclonal antibody reacts with CD158a
• CD158a is a 58 kDa membrane glycoprotein, member of the killer-inhibitory receptor (KIR)
• The KIR family family consists of transmembrane glycoproteins of the Ig superfamily expressed on <1-8% of NK cells and a subset of T cells which are involved in recognition of MHC class I molecules on target cells and inhibit cytotoxicity
• CD158a is an antigen which recognizes HLA-C molecules (-Cw*0401 and -Cw*1503)
• This antibody is suitable for staining acetone-fixed, frozen tissue sections
• It was reported that this clone also crossreacted with NKAT8 (CD158i) tranfectant cells
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.