CD52 Mouse anti-Human, Brilliant Violet 510, Clone: 4C8, BD

Description

• The 4C8 monoclonal antibody specifically binds to CD52 which is also known as Cambridge pathology 1 antigen (CAMPATH-1) or Human epididymis-specific protein 5 (HE5)
• CD52 is a highly N-glycosylated, 25-29 kDa protein whose C-terminus is glycophosphatidylinositol anchored in the membrane
• It is highly expressed on the surface of thymocytes and mature lymphocytes but not on their stem cell precursors
• It is also expressed on monocytes, dendritic cells, eosinophils and epithelial cells of the epididymis and seminal vesicles but not on neutrophils, plasma cells, platelets or erythrocytes
• Although its functional role is not well characterized, the CD52 antigen serves as an exquisitely sensitive target antigen for antibody and complement-mediated lysis of CD52-positive cells
• Anti-CD52 antibodies are being used clinically to remove lymphocytes from transplanted bone marrow cell preparations and in the treatment of some malignant diseases
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set
• BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.