CD71 Mouse anti-Human, Brilliant Violet 711, Clone: M-A712, BD

Description

• The M-A712 monoclonal antibody specifically binds to CD71 which is also known as the transferrin receptor (TFR)
• This type II transmembrane glycoprotein is expressed on cells as a disulfide-linked homodimer comprised of 95 kDa monomers
• CD71 is expressed on activated lymphocytes, monocytes, macrophages, erythroid progenitors, brain endothelium, and most proliferating cells
• CD71 is not expressed on resting lymphocytes and is upregulated during lymphocyte responses to antigens or mitogens
• Through an endocytic pathway, the transferrin receptor mediates cellular iron uptake by binding and internalizing iron that is bound to transferrin
• After releasing iron within the low pH endosomal environment, transferrin and its receptor can be recycled to the cell surface
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.