CD177 Mouse anti-Human, Brilliant Violet 421, Clone: MEM-166, BD

Description

• The MEM-166 monoclonal antibody specifically binds to CD177 which is also known as NB1 (Neutrophil-specific antigen), HNA-2a (Human neutrophil alloantigen 2a), and PRV1 (Polycythemia rubra vera 1)
• CD177 is a glycophosphatidylinositol (GPI)-anchored plasma membrane glycoprotein (56-64 kDa)
• CD177 is expressed on subpopulations of neutrophils, neutrophillic metamyelocytes and myelocytes from 89 to 97% of healthy individuals
• It is also expressed on secondary granules
• Its expression is upregulated on granulocytes stimulated with the chemotactic peptide f-met-leu-phe (fMLP)
• Although the function of CD177 has not been fully established, some reports suggest its possible role as receptor molecule involved in leucocyte migration
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.