CD278 Mouse anti-Human, Brilliant Violet 711, Clone: DX29, BD

Description

• The DX29 monoclonal antibody specifically binds to human CD278 which is also known as inducible costimulator (ICOS) and inducible T-cell co-stimulator
• ICOS is a homodimeric type I transmembrane glycoprotein with an approximate molecular weight of 50-60 kDa
• It is a member of the CD28 family and is highly expressed on activated T cells
• CD278 is the receptor for ICOS-ligand (also known as CD275, B7-H2, B7RP-1, and LICOS)
• Like CD28, ICOS can provide a costimulatory signal for T cell activation, proliferation and cytokine production
• It is not expressed on resting or activated B cells, monocytes, NK cells, granulocytes, dendritic cells or platelets
• Unlike the constitutively expressed CD28, ICOS is de novo expressed upon cellular activation
• Reports describe similarities between CD28 and ICOS in T cell activation, such as the costimulation of cytokine production
• However, it has been suggested that ICOS may play a greater role in IL-10 production
• In the presence of IL-10, purified recombinant human ICOS protein significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM) and enhanced production of IgG
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.