IFN-Gamma Rat anti-Mouse, Brilliant Violet 650, Clone: XMG1.2, BD

Description

• The XMG1.2 monoclonal antibody specifically binds to mouse interferon- γ(IFN- γ) protein
• IFN- γis a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses
• It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types
• IFN- γproduction is associated with the Th1 cell differentiation
• The purified form of this antibody has been reported to be a neutralizing antibody
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.