CD135 Mouse anti-Human, Brilliant Violet 711, Clone: 4G8, BD

Description

• The 4G8 monoclonal antibody specifically binds to CD135 which is also known as FMS-like tyrosine kinase 3 (FLT3), Fetal liver kinase 2 (FLK2) and Stem cell tyrosine kinase 1 (STK1)
• CD135 is a 155-160 kDa type I transmembrane glycoprotein
• It belongs to subclass III of the receptor tyrosine kinase (RTK) family which includes receptors for M-CSF (CD115), SCF/c-Kit Ligand (CD117), and PDGF (CD140a and CD140b)
• CD135 is expressed on multipotential, myelomonocytic and primitive B-cell progenitors
• The most primitive hematopoietic progenitor cells express low levels of CD135
• CD135 plays the role of growth factor receptor for early hematopoietic progenitors
• Reports from studies with knockout mice have shown that targeted CD135 disruption impaired the development of primitive progenitor cells of all hematopoietic lineages with significant impact on lymphopoietic precursors
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.