CXCR5 Rat anti-Mouse, BUV395, Clone: 2G8, BD

Description

• The 2G8 monoclonal antibody specifically binds to the mouse CXC chemokine receptor, CXCR5
• CXCR5 is also known as CD185, BLR1, NLR and MDR15
• CXCR5 is a seven-transmembrane, G-protein-coupled receptor that is specific for the CXC chemokine, CXCL13/BLC/BCA-1
• The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary
• In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes
• CXCR5 plays a role in directing the migration of B and T cells to B cell follicles with the spleen and certain other lymphoid tissues
• The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1)
• The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
• With an Ex Max at 348 nmand an Em Max at 395nm, this dye has virtually no spillover into any other detector
• BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.