KLRG1 Hamster anti-Mouse, Brilliant Violet 605, Clone: 2F1, BD

Description

• The 2F1 monoclonal antibody specifically binds to KLRG1 ( K iller cell L ectin-like R eceptor mmunoreceptor T yrosine-based I nhibitory M otif); its ligand has not been identified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa
• The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected
• Cross-linking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++ mobilization and cytotoxic responses (but not IFN- γproduction) by CD8+ T cells and inhibits IFN- γand TNF- α production and redirected lysis by NK cells
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and Cy™3.5
• Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.