CD80 Hamster anti-Mouse, Brilliant Violet 605, Clone: 16-10A1, BD

Description

• The 16-10A1 antibody reacts with CD80 (B7-1)
• This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4)
• CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig
• Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours
• It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours
• The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines
• However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells
• CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4)
• Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat
• No
• 553368)
• In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN- γ-activated alveolar macrophages of the dog
• This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.