CD86 Rat anti-Mouse, Brilliant Violet 605, Clone: GL1, BD

Description

• The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes
• CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture
• In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level
• CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions
• It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity
• CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4)
• GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells
• In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon™ BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon™ BV605 is a tandem fluorochrome of BD Horizon™ BV421 and Cy™3.5
• Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon™ PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon™ BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.