CD80 Hamster anti-Mouse, Brilliant Violet 650, Clone: 16-10A1, BD

Description

• The 16-10A1 antibody reacts with CD80 (B7-1)
• This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4)
• CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig
• Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours
• It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours
• The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines
• However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells
• CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4)
• Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat
• No
• 553368)
• In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN- γ-activated alveolar macrophages of the dog
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.