CD56 Mouse anti-Human, Brilliant Violet 650, Clone: NCAM16.2 (also known as NCAM 16), BD

Description

• The NCAM16.2 monoclonal antibody specifically binds to human CD56
• It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39
• The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes
• It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes
• CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity
• CD56 antigen density on NK lymphocytes increases upon cellular activation
• The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis
• CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.