CD39 Mouse anti-Human, Brilliant Violet 650, Clone: TU66 (also known as Tü 66, Tü66), BD

Description

• The TU66 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) an ectoenzyme that degrades ATP to AMP
• It is a member of the family of ectonucleoside triphosphate dihydrolases (E-NTPDases) known to be involved in regulation of extracellular nucleotide catabolism, controlling the extracellular nucleoside triphosphate pool (NTP)
• CD39 is expressed on a subset of T cells, B cells and dendritic cells with weak staining of monocytes and granulocytes
• Recently, CD39 has been found to be expressed primarly by immune-suppressive Foxp3(+) regulatory T (Treg) cells in both human and mice
• In humans, CD39 is restricted to a subset of Foxp3+ regulatory effector/memory-like T cells
• In mice, the enzyme is present on most if not all CD4+CD25+ cells and CD39 expression is driven by Foxp3
• It is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.