CD39 Mouse anti-Human, PE-CF594, Clone: TU66 (also known as Tü 66, Tü66), BD

Description

• The TU66 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) an ectoenzyme that degrades ATP to AMP
• It is a member of the family of ectonucleoside triphosphate dihydrolases (E-NTPDases) known to be involved in regulation of extracellular nucleotide catabolism, controlling the extracellular nucleoside triphosphate pool (NTP)
• CD39 is expressed on a subset of T cells, B cells and dendritic cells with weak staining of monocytes and granulocytes
• Recently, CD39 has been found to be expressed primarly by immune-suppressive Foxp3(+) regulatory T (Treg) cells in both human and mice
• In humans, CD39 is restricted to a subset of Foxp3+ regulatory effector/memory-like T cells
• In mice, the enzyme is present on most if not all CD4+CD25+ cells and CD39 expression is driven by Foxp3
• It is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).