CD134 Mouse anti-Human, Brilliant Violet 650, Clone: ACT35 (also known as Ber-ACT35), BD

Description

• The ACT35 monoclonal antibody specifically binds to CD134 which is also known as OX40
• The 35 kDa CD134 polypeptide is encoded by the TNFRSF4 gene
• CD134 is a type I integral membrane glycoprotein and member of the tumor necrosis factor/nerve growth factor receptor (TNFR/NGFR) family
• CD134 is expressed on activated T lymphocytes, hematopoietic precursor cells and fibroblasts
• CD134 functions as a T cell costimulatory receptor when bound by OX40 Ligand/TNFSF4 that is expressed by antigen presenting cells
• CD134 thereby plays roles in T-cell activation as well as the regulation of differentiation, proliferation or apoptosis of normal and malignant lymphoid cells
• Analysis of the nucleotide sequence of the human TNFRSF4 cDNA reveals strong homology with the rat Tnfrsf4 cDNA sequence
• OX40 was clustered as CD134 in the Sixth International Workshop on Human Leukocyte Differentiation Antigens
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.