CD335 (NKp46) Mouse anti-Human, Brilliant Violet 421, Clone: 9E2/Nkp46, BD

Description

• The 9E2/Nkp46 monoclonal antibody specifically binds to CD335
• CD335 is also known as the Natural killer cell p46-related protein (NKp46) and the Natural cytotoxicity triggering receptor 1 (NCR1)
• CD335 is a 46 kDa type I membrane glycoprotein that is expressed on resting and activated NK cells
• Its extracel lular region contains two C2-type, Ig-like domains
• The transmembrane domain contains a positively charged amino acid (Arg) which could be involved in stabilizing the association with CD3ζ
• Its intracellular region does not contain immunoreceptor tyrosine-based activating motifs (ITAM), but it is linked to intracytoplasmic transduction machinery by its association with CD3ζ and FcεRIγadaptor proteins
• CD335 along with NKp30 and NKp44 are referred to as natural cytotoxicity receptors (NCR)
• These receptors play very important roles in cells that kill virus-infected target cells, tumor cells and MHC-class I-unprotected cells
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.