CD54 Mouse anti-Human, Brilliant Violet 711, Clone: HA58, BD

Description

• The HA58 monoclonal antibody specifically binds to CD54 which is also known as ICAM-1 (intracellular adhesion molecule-1)
• CD54 is an 85-110 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin supergene family
• A soluble form of CD54 can also be found in biological fluids
• CD54 is expressed on endothelial cells and both resting (weak) and activated (moderate) lymphocytes and monocytes
• CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18)
• The CD54 adhesion molecule plays roles in inflammatory and immune responses and neoplasia
• This antibody (NA/LE format) blocks the mixed-lymphocyte reaction (MLR) and the purified format is suitable for staining acetone-fixed, frozen tissue sections
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.