CD158f Mouse anti-Human, Brilliant Violet 421, Clone: UP-R1, BD

Description

• The UP-R1 monoclonal antibody specifically recognizes CD158f which is also known as KIR2DL5
• CD158f is a ñ60 kDa single-pass type I transmembrane glycoprotein that belongs to the KIR (Killer cell Ig-like receptors) family within the Ig gene superfamily
• CD158f is encoded by two gene paralogs, KIR2DL5A and KIR2DL5B /that show greater than 99% identity in their coding sequences
• /CD158f molecules contain two extracellular C2-type Ig-like domains and a long cytoplasmic domain containing one canonical immunoreceptor tyrosine-based inhibitory motif (ITIM) and another atypical ITIM sequence
• Although the exact ligands for CD158f have not yet been identified, the inhibitory function of UP-R1 antibody-crosslinked CD158f was shown to primarily depend upon a Src homology region 2-containing protein tyrosine phosphatase-2 (SHP-2) -dependent pathway
• CD158f is highly polymorphic and is expressed on subsets of NK cells and T lymphocytes by some individuals
• UP-R1 recognizes cell surface CD158f encoded by the most commonly expressed allele, KIR2DL5A*001
• The UP-R1 antibody has been predicted to recognize CD158f molecules encoded by other transcriptionally-active KIR2DL5 alleles, A*012 and B*00602 The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.